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Characteristics of two transferable aminoglycoside resistance plasmids in

Chengjun Pu, Xiaoyan Gong, Ying Sun

Frontiers of Environmental Science & Engineering 2019, Volume 13, Issue 3, doi: 10.1007/s11783-019-1119-2

Abstract:

pRKZ3 is a non-conjugative IncQ plasmid, while pKANJ7 is a conjugative IncX plasmid.

The optimal mating time of pKANJ7 varied under different conditions.

Both of the two transferable ARPs had little impact on the growth of their hosts.

A relatively high level of fitness cost was observed for pKANJ7.

The fitness cost of ARPs depended on their hosts.

Keywords： <i>Escherichia colii>     Characteristics     Aminoglycoside resistance plasmids     Transfer     Persistence    

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Analysis of antibiotic resistance of

Yangyang Yu, Xiaolin Zhu, Guanlan Wu, Chengzhi Wang, Xing Yuan

Frontiers of Environmental Science & Engineering 2019, Volume 13, Issue 3, doi: 10.1007/s11783-019-1123-6

Abstract: >

There was a seasonal difference in antibiotic resistance of <i>E. colii>.

The <i>E. coli i>in the Yitong River was found

Keywords： Yitong River     Coliform bacteria     Antibiotic resistance     <i>Escherichia colii>    

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Effect of ultraviolet irradiation and chlorination on ampicillin-resistant

Yuchen PANG,Jingjing HUANG,Jinying XI,Hongying HU,Yun ZHU

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 3,   Pages 522-530 doi: 10.1007/s11783-015-0779-9

Abstract: Antibiotic resistance is a serious public health risk that may spread via potable and reclaimed water. Effective disinfection is important for inactivation of antibiotic-resistant bacteria and disruption of antibiotic resistance genes. Ampicillin is a widely prescribed antibiotic but its effectiveness is increasingly undermined by resistance. In this study, changes in ampicillin resistance for ( ) CGMCC 1.1595 were analyzed after exposure to different doses of ultraviolet (UV) or chlorine, and damage incurred by the plasmid encoding ampicillin resistance gene was assessed. We reported a greater stability in ampicillin-resistant CGMCC 1.1595 after UV irradiation or chlorination when compared with previously published data for other strains. UV irradiation and chlorination led to a shift in the mortality frequency distributions of ampicillin-resistant when subsequently exposed to ampicillin. The ampicillin hemi-inhibitory concentration (IC ) without disinfection was 3800 mg·L , and an increment was observed after UV irradiation or chlorination. The IC of ampicillin-resistant was 1.5-fold higher at a UV dose of 40 mJ·cm , and was 1.4-fold higher when exposed to 2.0 mg·L chlorine. These results indicate that UV irradiation and chlorination can potentially increase the risk of selection for strains with high ampicillin resistance. There was no evident damage to after 1–10 mg Cl ·L chlorination, while a UV dose of 80 mJ·cm yielded a damage ratio for of approximately 1.2-log. Therefore, high UV doses are required for effective disruption of antibiotic resistance genes in bacteria.

Keywords： antibiotic resistance     <i>Escherichia colii>     ampicillin resistance gene     ultraviolet irradiation     chlorination    

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Deposition of copper nanoparticles on multiwalled carbon nanotubes modified with poly (acrylic acid) and their antimicrobial application in water treatment

Li SHENG,Shuhang HUANG,Minghao SUI,Lingdian ZHANG,Lei SHE,Yong CHEN

Frontiers of Environmental Science & Engineering 2015, Volume 9, Issue 4,   Pages 625-633 doi: 10.1007/s11783-014-0711-8

Abstract: A novel hybrid material, Cu-PAA/MWCNTs (copper nanoparticles deposited multiwalled carbon nanotubes with poly (acrylic acid) as dispersant, was prepared and expected to obtain a more effective and well-dispersed disinfection material for water treatment. X-ray energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM), the X-ray fluorescence (XRF), X-ray photoelectron spectra (XPS), Fourier transform infrared spectra (FT-IR), Raman spectroscopy, and thermal gravimetric analyzer (TGA) were used to characterize the Cu-PAA/MWCNTs. ( ) was employed as the target bacteria. The cell viability determination and fluorescence imaging results demonstrated that Cu-PAA/MWCNTs possessed strong antimicrobial ability on . The deposited Cu was suggested to play an important role in the antimicrobial action of Cu-PAA/MWCNTs.

Keywords： multiwalled carbon nanotubes     copper nanoparticles     antimicrobial activity     <i>Escherichia colii> (<i>E. coili>)    

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Transmission of Carbapenem Resistance Between Human and Animal NDM-Positive <i>Escherichia colii> Strains Article

Yingbo Shen, Fupin Hu, Yongqiang Wang, Dandan Yin, Lu Yang, Yiqiang Chen, Chunyan Xu, Jiyun Li, Junyao Jiang, Xueyang Wang, Yulin Fu, Dongyan Shao, Dejun Liu, Tengfei Ma, Chang Cai, Zhangqi Shen, Shaolin Wang, Juan Li, Rong Zhang, Yuebin Ke, Congming Wu, Jianzhong Shen, Timothy R. Walsh, Yang Wang

Engineering 2022, Volume 15, Issue 8,   Pages 24-33 doi: 10.1016/j.eng.2021.07.030

Abstract: text-align: justify;">Although carbapenem use is prohibited in animals in China, carbapenem-resistant EscherichiaOverall, 631/29799 (2.1%) clinical Escherichia coli (E. coli) isolates were identified

Keywords： Carbapenem resistance     i>Escherichia colii>     <i>blai>NDM-5     One Health     Antimicrobial    

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Genetic and Functional Differences of <i>Escherichia colii> Strains from Colorectal Cancer Mucosal Article

Yuxiao Chang, Xiang Li, Lei Ding, Chao Yang, Zhiyuan Pan, Ni Han, Yujun Cui, Fachao Zhi, Ruifu Yang, Hong Gao, Yujing Bi

Engineering 2022, Volume 16, Issue 9,   Pages 210-219 doi: 10.1016/j.eng.2021.03.028

Abstract: Here, we used culturomics to isolate CRC mucosal samples and selected 158 Escherichia coli strains

Keywords： Colorectal cancer     Gut microbiota     Culturomics     Escherichia coli     Strain level    

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Detection of Escherichia coli in wastewater based on enzyme immunoassay

XI Haiyan, CAI Qiang, HE Miao, SHI Hanchang

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 3,   Pages 381-384 doi: 10.1007/s11783-007-0065-6

Abstract: This research describes a fast detection method on the basis of enzyme-linked immunosorbent assay (ELISA) for in drainage of wastewater treatment plants. Optimized conditions such as the reaction format (sandwich or direct), the concentrations of diluted horse radish peroxidase (HRP)- conjugate, and anti-HPR antibody and pretreatment of were studied. Those results showed that the linear range of detection for was 10 cfu/mL– 6 × 10 cfu/mL. Compared with conventional methods, it is a convenient and sensitive detection method with low cost.

Keywords： conventional     sandwich     pretreatment     enzyme-linked immunosorbent     detection    

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italic>rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of Escherichia

Prayoga SURYADARMA, Yoshihiro OJIMA, Yuto FUKUDA, Naohiro AKAMATSU, Masahito TAYA

Frontiers of Chemical Science and Engineering 2012, Volume 6, Issue 2,   Pages 152-157 doi: 10.1007/s11705-012-1287-0

Abstract: The role of on the central carbon metabolism was investigated through analyzing the deficiency of this regulon gene under aerobic and glucose-enriched culture conditions. The experimental results showed that while the wild type cells exhibited an overflow metabolism effect, the -deleting mutation alleviated this effect with the significant suppression of acetate accumulation under a high glucose condition. This gene deletion also induced the twofold upregulation of and one-tenth downregulation of , respectively. The overflow metabolism effect was confirmed to be recovered by re-introducing gene into the mutant. These results demonstrated changed the central carbon metabolism toward acetate overflow through dehydrogenation of pyruvate and reduction of TCA cycle activity.

Keywords： Escherichia coli     rpoS     aerobic and glucose-enriched culture     overflow    

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A One-Health Sampling Strategy to Explore the Dissemination and Relationship Between Colistin Resistance in Human, Animal, and Environmental Sectors in Laos Article

Yuqing Zhou, Refath Farzana, Somsavanh Sihalath, Sayaphet Rattanavong, Manivanh Vongsouvath, Mayfong Mayxay, Kirsty Sands, Paul N. Newton, David A.B. Dance, Brekhna Hassan, Timothy R. Walsh

Engineering 2022, Volume 15, Issue 8,   Pages 45-56 doi: 10.1016/j.eng.2022.01.013

Abstract: A total of 238 Escherichia coli (E. coli) isolated from nonduplicative samples, consisting

Keywords： Colistin resistance     <i>mcri>     <i>Escherichia colii> Laos     Horizontal transmission    

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Biomimetic Macrophage–Fe3O4@PLGA Particle-Triggered Intelligent Catalysis for Killing Multidrug-Resistant Escherichia

Jieni Fu,Xiangmei Liu,Zhaoyang Li,Yufeng Zheng,Yu Zhang,Hui Jiang,Yanqin Liang,Shengli Zhu,Zhenduo Cui,Shuilin Wu,

Engineering doi: 10.1016/j.eng.2023.05.022

Abstract: Infections with multidrug-resistant (MRD) Gram-negative bacteria, such as MRD Escherichia coli3O4@PLGA particles (i.e., the intelligent catalysis particles) exhibit

Keywords： Multidrug-resistant Escherichia coli     Macrophage–Fe3O4@PLGA particles     Biomimetic intelligent catalysis    

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Enhanced disinfection of Escherichia coli and bacteriophage

Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 4, doi: 10.1007/s11783-016-0854-x

Abstract: A novel photocatalytic Ag-Cu-TiO nanowire membrane was fabricated. Bacteria and virus disinfection was improved by co-depositing Ag and Cu onto membrane. Synergetic photocatalytic effects and free metal ions of Ag and Cu contribute to disinfection. 7.68 log removal of and 4.02 log removal of bacteriophage MS2 were achieved. Titanium dioxide (TiO ) is a widely used photocatalyst that has been demonstrated for microorganism disinfection in drinking water. In this study, a new material with a novel structure, silver and copper loaded TiO nanowire membrane (Cu-Ag-TiO ) was prepared and evaluated for its efficiency to inactivate and bacteriophage MS2. Enhanced photo-activated bactericidal and virucidal activities were obtained by the Cu-Ag-TiO membrane than by the TiO , Ag-TiO and Cu-TiO membranes under both dark and UV light illumination. The better performance was attributed to the synergies of enhanced membrane photoactivity by loading silver and copper on the membrane and the synergistic effect between the free silver and copper ions in water. At the end of a 30 min test of dead-end filtration under 254 nm UV irradiation, the Cu-Ag-TiO membrane was able to obtain an removal of 7.68 log and bacteriophage MS2 removal of 4.02 log, which have met the US EPA standard. The free metal ions coming off the membrane have concentrations of less than 10 ppb in the water effluent, far below the US EPA maximum contaminant level for silver and copper ions in drinking water. Therefore, the photo-activated disinfection by the Cu-Ag-TiO membrane is a viable technique for meeting drinking water treatment standards of microbiological water purifiers.

Keywords： Photo-activated disinfection     Titanium dioxide     Nanowire membrane     Silver     Copper    

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Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

Frontiers of Structural and Civil Engineering 2014, Volume 8, Issue 4,   Pages 448-455 doi: 10.1007/s11709-014-0262-x

Abstract: Rock mass classification system is very suitable for various engineering design and stability analysis. classification method is confirmed by Japan Highway Public Corporation that this method can figure out either strength or deformability of rock mass, further appropriating the amount of rock bolts, thickness of shotcrete, and size of pitch of steel ribs just after the blasting procedure. Based on these advantages of method, in this study, according to data of five deep and long tunnels in Iran, two equations for estimating the value of method from and classification systems were developed. These equations as a new method were able to optimize the support system for and classification systems. From classification and its application in these case studies, it is pointed out that the method for the design of support systems in underground working is more reliable than the and classification systems.

Keywords： <i>JHi> classification     <i>Qi> and <i>RMRi> classification     new method    

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The Potential Role of Powdery Mildew-Resistance Gene <i>Pm40i> in Chinese Wheat-Breeding Programs inthe Post-<i>Pm21i> Era Perspective

Shengwen Tang,Yuting Hu,Shengfu Zhong,Peigao Luo

Engineering 2018, Volume 4, Issue 4,   Pages 500-506 doi: 10.1016/j.eng.2018.06.004

Abstract:

Powdery mildew, which is caused by Blumeria graminis f. sp. tritici (Bgt), is an important leaf disease that affects wheat yield. Powdery mildew-resistance (Pm) gene Pm21 was first transferred into wheat in the 1980s, by translocating the Heuchera villosa chromosome arm 6VS to the wheat chromosome arm 6AL (6VS·6AL). Recently, new Bgt isolates that are virulent to Pm21 have been identified in some wheat fields, indicating that wheat breeders should be aware of the risk of deploying Pm21, although pathological details regarding these virulent isolates still remain to be discovered. Pm40 was identified and mapped on the wheat chromosome arm 7BS from several wheat lines developed from the progenies of a wild cross between wheat and Thinopyrum intermedium. Pm40 offers a broad spectrum of resistance to Bgt, which suggests that it is likely to provide potentially durable resistance. Cytological methods did not detect any large alien chromosomal segment in the wheat lines carrying Pm40. Lines with Pm40 and promising agronomical traits have been released by several wheat-breeding programs in the past several years. Therefore, we believe that Pm40 will play a role in powdery mildew-resistance wheat breeding after Pm21 resistance is overcome by Bgt isolates. In addition, both Pm21 and Pm40 were derived from alien species, suggesting that the resistance genes derived from alien species are potentially more durable or effective than those identified from wheat.

Keywords： Wheat     Powdery mildew     <i>Pm21i>     <i>Pm40i>     Alien species     Native resistance    

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NiFeRuO<i>xi> nanosheets on Ni foam as an electrocatalyst for efficient

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 11,   Pages 1698-1706 doi: 10.1007/s11705-023-2334-8

Abstract: The electrocatalyst NiFeRuO<i>xi>/NF, comprised of NiFeRuO<i>xi> nanosheetsNiFeRuO<i>xi>/NF displays high electrocatalytic activity and stability for overall alkalineA solar-driven system containing such a bifunctional NiFeRuO<i>xi>/NF has an almost 100%The NiFeRuO<i>xi> coating around Ni foam is an anti-corrosion layer and also a critical

Keywords： NiFeRuO<i>xi> nanosheets     Ni foam     electrocatalysis     overall seawater splitting     solar-driven    

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Presence and Antimicrobial Susceptibility of RE-<i>cmeABCi>-Positive <i>Campylobacteri> Isolated Article

Dejun Liu, Weiwen Liu, Xing Li, Hong Yao, Zhangqi Shen, Yang Wang, Jianzhong Shen

Engineering 2020, Volume 6, Issue 1,   Pages 34-39 doi: 10.1016/j.eng.2019.10.013

Abstract:

Campylobacter spp. (C. spp.) are the leading cause of human gastroenteritis worldwide. RE-CmeABC is a newly identified resistance-enhancing multidrug efflux pump of C. spp. that confers high-level resistance to fluoroquinolones, phenicols, macrolides, and tetracyclines, all of which are critical drugs in both human and veterinary medicine. In this study, we analyzed the presence and antimicrobial susceptibility of RE-cmeABC-positive Campylobacter isolates of food-animal origin from three representative regions (Shandong, Shanghai, and Guangdong) in China over three successive years, from 2014 to 2016. A total of 1088 Campylobacter isolates (931 C. coli and 157 C. jejuni) were recovered from the RE-cmeABC screening. We detected 122 (11.2%) RE-cmeABC-positive isolates of chicken origin, including 111 (70.7%) C. jejuni and 11 (1.2%) C. coli. This multidrug efflux pump is more prevalent among C. jejuni than C. coli. The level of resistance was significantly different in 111 RE-cmeABC-positive C. jejuni versus 46 RE-cmeABC-negative C. jejuni for florfenicol, clindamycin, and erythromycin (P < 0.05), but not for ciprofloxacin (CIP), tetracycline (TET), and gentamicin. However, the isolates harboring RE-cmeABC could shift the minimum inhibitory concentration distribution to the higher range for CIP and TET. Pulsed-field gel electrophoresis (PFGE) analysis suggested that horizontal transmission might be involved in the dissemination of RE-cmeABC in Shanghai and Guangdong, while clonal expansion was predominant in Shandong. Three isolates shared the indiscriminate PFGE types of RE-cmeABC-positive C. jejuni isolates in Shanghai and Guangdong, and four isolates in Shanghai and Shandong. Our study suggests the possibility of a wide dissemination of RE-cmeABC in Campylobacter of food-animal origin, which would pose a significant threat to public health.

Keywords： RE-<i>cmeABCi>     <i>Campylobacteri>     Ciprofloxacin     Florfenicol    

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Title Author Date Type Operation

Characteristics of two transferable aminoglycoside resistance plasmids in

Chengjun Pu, Xiaoyan Gong, Ying Sun

Journal Article

Analysis of antibiotic resistance of

Yangyang Yu, Xiaolin Zhu, Guanlan Wu, Chengzhi Wang, Xing Yuan

Journal Article

Effect of ultraviolet irradiation and chlorination on ampicillin-resistant

Yuchen PANG,Jingjing HUANG,Jinying XI,Hongying HU,Yun ZHU

Journal Article

Deposition of copper nanoparticles on multiwalled carbon nanotubes modified with poly (acrylic acid) and their antimicrobial application in water treatment

Li SHENG,Shuhang HUANG,Minghao SUI,Lingdian ZHANG,Lei SHE,Yong CHEN

Journal Article

Transmission of Carbapenem Resistance Between Human and Animal NDM-Positive <i>Escherichia colii> Strains

Yingbo Shen, Fupin Hu, Yongqiang Wang, Dandan Yin, Lu Yang, Yiqiang Chen, Chunyan Xu, Jiyun Li, Junyao Jiang, Xueyang Wang, Yulin Fu, Dongyan Shao, Dejun Liu, Tengfei Ma, Chang Cai, Zhangqi Shen, Shaolin Wang, Juan Li, Rong Zhang, Yuebin Ke, Congming Wu, Jianzhong Shen, Timothy R. Walsh, Yang Wang

Journal Article

Genetic and Functional Differences of <i>Escherichia colii> Strains from Colorectal Cancer Mucosal

Yuxiao Chang, Xiang Li, Lei Ding, Chao Yang, Zhiyuan Pan, Ni Han, Yujun Cui, Fachao Zhi, Ruifu Yang, Hong Gao, Yujing Bi

Journal Article

Detection of Escherichia coli in wastewater based on enzyme immunoassay

XI Haiyan, CAI Qiang, HE Miao, SHI Hanchang

Journal Article

italic>rpoS deficiency suppresses acetate accumulation in glucose-enriched culture of Escherichia

Prayoga SURYADARMA, Yoshihiro OJIMA, Yuto FUKUDA, Naohiro AKAMATSU, Masahito TAYA

Journal Article

A One-Health Sampling Strategy to Explore the Dissemination and Relationship Between Colistin Resistance in Human, Animal, and Environmental Sectors in Laos

Yuqing Zhou, Refath Farzana, Somsavanh Sihalath, Sayaphet Rattanavong, Manivanh Vongsouvath, Mayfong Mayxay, Kirsty Sands, Paul N. Newton, David A.B. Dance, Brekhna Hassan, Timothy R. Walsh

Journal Article

Biomimetic Macrophage–Fe3O4@PLGA Particle-Triggered Intelligent Catalysis for Killing Multidrug-Resistant Escherichia

Jieni Fu,Xiangmei Liu,Zhaoyang Li,Yufeng Zheng,Yu Zhang,Hui Jiang,Yanqin Liang,Shengli Zhu,Zhenduo Cui,Shuilin Wu,

Journal Article

Enhanced disinfection of Escherichia coli and bacteriophage

Guiying RAO, Kristen S. BRASTAD, Qianyi ZHANG, Rebecca ROBINSON, Zhen HE, Ying LI

Journal Article

Development of a new method for RMR and Q classification method to optimize support system in tunneling

Asghar RAHMATI,Lohrasb FARAMARZI,Manouchehr SANEI

Journal Article

The Potential Role of Powdery Mildew-Resistance Gene <i>Pm40i> in Chinese Wheat-Breeding Programs inthe Post-<i>Pm21i> Era

Shengwen Tang,Yuting Hu,Shengfu Zhong,Peigao Luo

Journal Article

NiFeRuO<i>xi> nanosheets on Ni foam as an electrocatalyst for efficient

Journal Article

Presence and Antimicrobial Susceptibility of RE-<i>cmeABCi>-Positive <i>Campylobacteri> Isolated

Dejun Liu, Weiwen Liu, Xing Li, Hong Yao, Zhangqi Shen, Yang Wang, Jianzhong Shen

Journal Article